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recombinant human granulocyte macrophage colony  (R&D Systems)


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    R&D Systems recombinant human granulocyte macrophage colony
    Recombinant Human Granulocyte Macrophage Colony, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 546 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+granulocyte+colony/Recombinant+Human+GM-CSF+Protein/pmc13089343-221-21-26
    Average 96 stars, based on 546 article reviews
    recombinant human granulocyte macrophage colony - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Recombinant factor VIII expression in hematopoietic cells following lentiviral transduction.
    Article Snippet: Human PB-derived CD34þ cells obtained from two healthy donors were enriched to 498% using anti-CD34 microbeads (CliniMACS, Miltenyi) as described.33 CD34þ cells were cultured either in X-VIVO/ 10 containing 1% HSA or in RPMI 1640 containing 10% heat-inactivated FCS and 5 mM L-glutamine. .. As indicated, media were supplemented with human recombinant granulocyte colony-stimulating factor (G-CSF, 20 ng/ml, Amgen, Munich, Germany), granulocyte macrophage colony-stimulating factor (GM-CSF, 20 ng/ ml, Novartis, Basel, Switzerland), stem cell factor (SCF, 100 ng/ml, Amgen), thrombopoietin (TPO, 100 ng/ml, R&D Systems, Wiesenbaden-Nordenstadt, Germany), erythropoietin (Epo, 2.5 U/ml, Roche Diagnostics), Flt-3 ligand (Flt-3L, 100 ng/ml, Genzyme, Alzenau, Germany), IL-3 (10 ng/ml, Roche Diagnostics), and IL-6 (20 ng/ml, Roche Diagnostics). .. Hematopoietic cells were seeded at 0.5 to 2 106 per ml in serum-free X-VIVO/10 supplemented with 1% HSA, 200 mM dNTP and 4 mg/ml protamine sulfate in 24-well dishes coated with CH-296 (Retronectin; Takara Shuzo, Japan) as indicated.



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